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Products / Ladders / SciPhiTM 100 bp DNA Ladder – Ready to use

SciPhiTM 100 bp DNA Ladder - Ready to use

SciPhiTM 100 bp DNA Ladder - Ready to use
Catalog Number
  • NXG121–L (RTU)     : 500 Applications
  • NXG121-M (RTU)     : 100 Applications
  • NXG121-S (RTU)      : 50 Applications
  • NXG121-XS (RTU)   : 10 Applications
  • NXG120–L                 : 500 Applications
  • NXG120-M                 : 100 Applications
  • NXG120-S                   : 50 Applications
  • NXG120-XS                : 10 Applications

Product Description

  • Accurate DNA Sizing: SciPhi™ 100 bp DNA Ladder, is ready-to-use for precise sizing and approximate quantification of a diverse range of double-stranded DNA on agarose gels.
  • Band Pattern: Ten highly purified DNA fragments ranging from 100 to 1000 base pairs with a difference of 100 bp.
  • Reference Band: Includes a reference band at 500 bp for simplified orientation.
  • Ready to Use Format: Pre-mixed with 6X DNA Loading Dye for direct loading onto the gel, saves time.
  • SciPhi™ 6X Loading Dye: Supplied with 6X Loading dye that can be used for DNA samples/ PCR amplifications.
  • Applications:
    • Sizing standards
    • Approximate quantification
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Loading and Storage Buffer Composition:
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005 % bromophenol blue, 0.005 % xylene cyanol FF, 0.025 % orange G and 10 % glycerol.

6X DNA Loading Dye Composition:
10 mM Tris-HCl (pH 7.6), 0.03 % bromophenol blue, 0.03 % xylene cyanol FF, 0.15 % orange G, 60 % glycerol and 60 mM EDTA.

Instruction for loading DNA ladder:
Mix gently and load 1 μL per 1 mm gel lane.

Suggestions & Recommendations

  • Do not heat before loading.
  • Dilute your DNA sample with the 6X DNA Loading Dye (Cat#NXG112, supplied with the ladder: mix 1 volume of the dye solution with 5 volumes of the DNA sample.
  • Load the same volumes of the DNA sample and the DNA ladder.
  • For quantification, adjust the concentration of the sample to equalize it approximately with the amount of DNA in the nearest band of the ladder.
  • For DNA band visualization with SYBRTM Green and other intercalating dyes, do not add the dyes into the sample, use gel staining after electrophoresis or include dyes into agarose gel to avoid aberrant DNA migration.