SciPhi^TM Plasmid Miniprep Kit

Catalog Number

• NXG993-L      :    250 Preps
• NXG993-M     :    50 Preps
• NXG993-S      :     10 Preps
• NXG993-XS   :     05 Preps

Product Description

• Principle: Lyse, bind, wash and elute plasmid from bacterial culture.
• Silica Membrane Spin Column Technology: Kit utilizes silica membrane spin column technology for efficient plasmid DNA isolation. Fast: Purify high quality Plasmid DNA in less than 15 minutes.
• High Yields: Yield of 20 μg of high-quality plasmid DNA can be obtained.
• SciPhi^TM Purification Columns: Preassembled with collection tubes eliminate the need of phenol/chloroform extraction.
• Downstream Applications: Isolated plasmid DNA can be used for various downstream applications such as restriction digestion, automated fluorescent and radioactive sequencing, PCR, in vitro transcription, and transformation.

Storage Instructions:
SciPhi^TM Plasmid Mini prep Kit can be stored at room temperature (15 to 25°C) for upto 12 months. However, the kit can be kept at 4°C in case of storage periods is more than 1 year. Please remember to properly close the bag containing SciPhi^TM Genomic DNA Purification Columns after each usage.

Principle:

• Resuspension and Lysis: Bacterial cells in pellet form are revitalized and exposed to SDS/alkaline lysis to free the plasmid DNA.
• Neutralization: The resulting suspension’s pH is adjusted to neutral, providing the best environment for plasmid DNA attachment to the silica membrane of the column.
• Removal of cell debris and Binding: Cell debris and SDS precipitate are then pelleted by centrifugation, enabling the efficient binding of plasmid DNA present in supernatant, with silica membrane of the column.
• Washings: The bound plasmid DNA undergoes a purification/washing process with wash buffer to effectively remove impurities, ensuring the isolation of high-quality plasmid DNA.
• Elution: The purified plasmid DNA is then eluted using an Elution Buffer.