SciPhi^TM DNA Ligase

Catalog Number

• NXG661-L       :           40000 Units
• NXG661-M      :           20000 Units
• NXG661-S       :           10000 Units

Product Description

• Phosphodiester Bond Formation: Catalyses the creation of phosphodiester bonds between 3’-hydroxyl termini and adjacent 5’-Phosphate in 30 minutes at Room Temperature.
• Ligation Reaction: Efficient for both blunt end and sticky end ligation.
• Repairing Efficiency: Can repair single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.
• Applications:
  • Ligation of restriction enzyme digested fragments
  • Linking oligonucleotide linkers to DNA
  • Site-directed mutagenesis
  • AFLP
  • Repairing of DNA and RNA nicks
  • Facilitation the self-circularization of linear DNA.

Storage Conditions
Keep the item stored at a temperature of -20°C until it is ready for use. It is important to avoid subjecting the item to repeated freeze-thaw cycles.