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Products / Ladders / SciPhiTM 1 kb DNA Ladder – Ready to use

SciPhiTM 1 kb DNA Ladder - Ready to use

SciPhiTM 1 kb DNA Ladder - Ready to use
Catalog Number – NXG111

Product Description

  • SciPhiTM 1 kb DNA Ladder: Unparalleled precision in DNA sizing and approximate quantification on agarose gel with SciPhiTM 1 kb DNA Ladder.
  • Extensive Range: Fourteen chromatographic purified DNA fragments of wide range from 250 bp to 10,000 bp, enabling unmatched resolution and accuracy in your experiments.
  • Reference Bands: Three strategically positioned reference bands (1000, 3000, and 6000 bp), facilitating effortless orientation and ensuring reliable interpretation of your results.
  • Ready to Use: Premixed with 6X DNA Loading Dye, eliminating the need for time-consuming preparation steps. Simply load 1 μl per 1 mm Gel lane and save your valuable time and effort.
  • SciPhiTM 6X Loading Dye: Supplied with 6X loading dye(Cat#NXG112) that can be used to load DNA samples.
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Cat. No. Product Description Volume Storage
NXG111 SciPhiTM 1 kb DNA Ladder 5 x 50 μg for 500 applications Store at room temperature or at 4°C for periods up to 6 months. For longer periods, store at – 20°C
6X DNA Loading Dye 2 x 1 ml

Loading and Storage Buffer Composition:
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005 % bromophenol blue, 0.005 % xylene cyanol FF, 0.025 % orange G and 10 % glycerol.

6X DNA Loading Dye Composition:
10 mM Tris-HCl (pH 7.6), 0.03 % bromophenol blue, 0.03 % xylene cyanol FF, 0.15 % orange G, 60 % glycerol and 60 mM EDTA.

Instruction for loading DNA ladder:
Mix gently and load 1 μL per 1 mm gel lane.

Suggestions & Recommendations

  • Do not heat before loading.
  • Dilute your DNA sample with the 6X DNA Loading Dye (Cat#NXG112, supplied with the ladder: mix 1 volume of the dye solution with 5 volumes of the DNA sample.
  • Load the same volumes of the DNA sample and the DNA ladder.
  • For quantification, adjust the concentration of the sample to equalize it approximately with the amount of DNA in the nearest band of the ladder.
  • For DNA band visualization with SYBRTM Green and other intercalating dyes, do not add the dyes into the sample, use gel staining after electrophoresis or include dyes into agarose gel to avoid aberrant DNA migration.