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SciPhiTM RNA Purification Kit

SciPhiTM RNA Purification Kit
Catalog Number – NXG992

Product Description

  • Universal: SciPhiTM RNA Purification Kit can purify total RNA from bacteria, yeast, insects, tissue, human blood cells and mammalian cultured cells.
  • Silica Membrane Spin Column Technology: The kit is based on silica membrane spin column technology. Hence, no tedious cesium chloride gradients, no alcohol precipitation and no phenol-chloroform extraction.
  • Fast Protocol: Total RNA can be purified within 15 minutes after lysis for RNA molecule more than 200 nucleotides.
  • High Yield: SciPhiTM RNA Purification Kit can yield up to 10-160 μg total RNA as per sample type.
  • RNA Integrity / Purity: RNA samples with 260/280 ratio more than 1.9 can be extracted.
  • Downstream Applications: The high-quality purified RNA can be used for diverse range of downstream applications, including cDNA preparation, Real Time PCR, Quantitative Real Time PCR, Northern blotting, microarray analysis and other assays based on RNA.
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Storage Instructions:
As long as proteinase K solution is not opened, it is stable at room temperature. Once it has been opened, it needs to be kept at -20 °C. Other components of the kit can be kept at room temperature (15 to 25°C).

Please remember to properly close the bag containing SciPhiTM RNA Purification Columns after each use.

Digestion/Lysis: Depending on the initial material, the samples are subjected to Lysis Buffer. The lysis buffer contains guanidine thiocyanate which is capable to protect RNA from RNases.

Binding and Purification: The lysate (resulting mixture) is then mixed with ethanol and loaded onto a purification column. In this step, the RNA molecules present in the lysate attach to the silica membrane within the column, while impurities are effectively washed away using prepared wash buffers.

Elution: Total RNA is then can be eluted from the purification column under low ionic strength conditions using an Elution Buffer. This allows the RNA to be collected in a separate solution for further analysis or use.