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SciPhiTM qPCR Master Mix (2x)

SciPhiTM qPCR Master Mix (2x)
Catalog Number – NXG444

Product Description

  • Optimized 2X qPCR Master Mix: SciPhiTM qPCR Master Mix (2x) is an optimized and universal 2X master mix designed to amplify target genes with high sensitivity, enabling accurate and reliable gene expression analysis.
  • Template Volume: Accurate gene expression analysis by using 1–10 ng of single-stranded cDNA or 10–100 ng of genomic DNA (gDNA) per reaction.
  • Excellent specificity: Excellent specificity due to innovative dual hot- start mechanism incorporated into SciPhiTM qPCR Master Mix (2x).
  • Broad Dynamic Range: Consistent and reliable quantification of target genes with highly reproducible cycle threshold (CTs) values over a broad dynamic range.
  • Premixed with UDG: Prevent carryover contamination with the inclusion of Uracil-DNA Glycosylase (UDG) in SciPhiTM qPCR Master Mix Extended
  • Stability: Pre-assembled reactions with SciPhiTM qPCR Master Mix can remain stable for up to 72 hours.
  • Compatibility: Compatible to be used for standard/fast cycling mode with Applied Biosystems Real Time, BioRad IQ5 CFX384, Roche Light Cycler LC480 and Agilent/Stratagene MX3005P Real time machines.
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Cat. No. Product Description Volume Storage
NXG444 qPCR Master Mix ( 2x ) 5ml 2°C – 8°C


  • Highly reproducible CTs over a broad dynamic range.
  • Inclusion of UDG to help prevent carryover contamination.
  • ROXTM dye Passive Reference
  • Stability of pre-assembled reactions for up to 72 hours.
  • Compatibility with most real-time qPCR instruments.