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SciPhiTM Genomic DNA Purification Kit

SciPhiTM Genomic DNA Purification Kit
Catalog Number – NXG991

Product Description

  • Universal : SciPhiTM Genomic DNA Purification Kit is suitable for fast and efficient genomic DNA purification from whole blood, mammalian cell culture, tissue samples, bacteria and yeast.
  • Silica Membrane Spin Column Technology : The kit is based on silica membrane spin column technology. Hence, no resins, no phenol- chloroform extractions, and no alcohol precipitations are needed.
  • Fast Protocol : Efficient DNA purification can be done in less than 20 minutes after lysis.
  • HighYield : Yield of >30μg can be obtained with the kit.
  • Column Assembly : Capped spin columns assembled with collection tubes.
  • Downstream Applications : Purified genomic DNA from the kit is ideal for PCR, qPCR, restriction digestion, and Southern blotting applications.
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Storage Instructions:
As long as the RNase A and proteinase K solutions are not opened, they are stable at room temperature. Once they’ve been opened, they need to be kept at -20 °C. Other components of the kit can be kept at room temperature (15 to 25°C).

Please remember to properly close the bag containing SciPhiTM Genomic DNA Purification Columns after each usage.


Digestion/Lysis: Depending on the initial material, the samples are subjected to Proteinase K digestion using either the Digestion or Lysis Solution provided. This process helps break down the cells.

RNA Removal: To eliminate RNA present in the samples, they are treated with RNase A. This enzymatic treatment specifically targets and degrades RNA molecules.

Binding and Purification: The lysate (resulting mixture) is then mixed with ethanol and loaded onto a purification column. In this step, the DNA molecules present in the lysate attach to the silica membrane within the column, while impurities are effectively washed away using prepared wash buffers.

Elution: Genomic DNA is then can be eluted from the purification column under low ionic strength conditions using an Elution Buffer. This allows the DNA to be collected in a separate solution for further analysis or use.