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Products / Ladders / SciPhiTM Prestained Protein Ladder – Broad Range

SciPhiTM Prestained Protein Ladder - Broad Range

SciPhiTM Prestained Protein Ladder - Broad Range
Catalog Number – NXG222

Product Description

  • Broad Range: SciPhiTM Prestained Protein Ladder consisting of a mixture of nine recombinant proteins ranging from 10 kDa to 250 kDa to be used in SDS-PAGE and Western Blotting.
  • Brightly Coloured Ladder: Visualize protein bands easily with four different chromophores bound to the proteins, resulting in a brightly colored ladder for easy identification.
  • Ready-to-Use: Simplify your workflow with prestained protein ladder, eliminating the need for heating, dilution, or addition of reducing agents. Low lot-to-lot Variation: Consistent, reliable protein size measurements with ~5% lot-to-lot variation in apparent molecular weight.
  • Western Blotting Compatibility: Easily observe the protein transfer onto membranes after western blotting.
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Cat. No. Product Description Volume Storage
NXG222 SciPhiTM Prestained Protein Ladder – Broad Range 2 x 250 μl For 100 Applicatio ns Store at -20°C

Storage buffer:
62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Recommendations for Loading

  • Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids.
  • Do not boil.
  • Mix gently, but thoroughly, to ensure the solution is homogeneous.
  • Load the following volumes of the ladder on an SDS-polyacrylamide gel: – 5 μL per well for mini gel, – 10 μL per well for large gel.
  • Use the same volumes for Western blotting.
  • The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm.
  • The loading volume should be doubled for 1.5 mm thick gels.

Important Notes

  • Do not boil the protein ladder.
  • The SciPhiTM Prestained Protein Ladder (Broad Range) can be stored at 4 °C for up to 3 months.
  • Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system.
  • In low-percentage gels (< 10 %), the low- molecular weight proteins in the ladder may migrate with the dye front. The SciPhiTM Prestained Protein Ladder (Broad Range) can be used in Western blotting with all common membranes: PVDF, nylon and nitrocellulose. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100 kDa) proteins.